An unknown microorganism was isolated from the soil by using defined selective medium. It could synthesize glutamic acid from glucose and urea. When subjected to repeated ultra-violet light and X-ray irradiations, and after process of screening, the organism had increased the yield of glutamic acid biosynthesis up to 10 mgm per ml of broth. The pathway of glutamic acid biosynthesis is unknown. Evidence indicated that the TCA cycle was probably not involved. The microorganism could be a new species of Micrococcus.