Biology & Sustainability

Event Title

Intraspecific Variation in a Hawaiian Population of Ophioderma pendula (Ophioglossaceae)

Presenter Information

Carla Ramirez, Denison University

Document Type

Oral Presentation

Location

Indianapolis, IN

Subject Area

Biology & Sustainability

Start Date

11-4-2014 9:45 AM

End Date

11-4-2014 10:30 AM

Description

Long-distance dispersal is the major means by which organisms colonize islands. Colonization of islands requires that species possess unique traits that facilitate rare, long-distance dispersal events for the establishment of populations. Populations established by a single, long-distance dispersal event typically contain relatively low amounts of genetic variation. Ophioderma pendula (L.) Presl is an epiphytic fern (a plant that grows on trees) in the adder's tongue fern family (Ophioglossaceae) that has colonized successfully many islands distributed across the Indian Ocean and Pacific Oceans. To evaluate within-population genetic variation of O. pendula from Hawaii, I analyzed nuclear ISSR variation from 30 individual sporophytes, and two chloroplast DNA (cpDNA) regions (trnH-psbA, rpoB-trnC) of five individuals from a single population. Analysis of ISSR variation from seven primers revealed 26 multi-locus genotypes among the 30 samples. However, sequences for both cpDNA regions showed no variation across the five individuals sampled. Comparisons to cpDNA sequences from a sample of O. pendula from the Philippines are consistent with the hypothesis that this Hawaiian population originated from a single colonization event followed by mutation in ISSR nuclear DNA but not in cpDNA.

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Apr 11th, 9:45 AM Apr 11th, 10:30 AM

Intraspecific Variation in a Hawaiian Population of Ophioderma pendula (Ophioglossaceae)

Indianapolis, IN

Long-distance dispersal is the major means by which organisms colonize islands. Colonization of islands requires that species possess unique traits that facilitate rare, long-distance dispersal events for the establishment of populations. Populations established by a single, long-distance dispersal event typically contain relatively low amounts of genetic variation. Ophioderma pendula (L.) Presl is an epiphytic fern (a plant that grows on trees) in the adder's tongue fern family (Ophioglossaceae) that has colonized successfully many islands distributed across the Indian Ocean and Pacific Oceans. To evaluate within-population genetic variation of O. pendula from Hawaii, I analyzed nuclear ISSR variation from 30 individual sporophytes, and two chloroplast DNA (cpDNA) regions (trnH-psbA, rpoB-trnC) of five individuals from a single population. Analysis of ISSR variation from seven primers revealed 26 multi-locus genotypes among the 30 samples. However, sequences for both cpDNA regions showed no variation across the five individuals sampled. Comparisons to cpDNA sequences from a sample of O. pendula from the Philippines are consistent with the hypothesis that this Hawaiian population originated from a single colonization event followed by mutation in ISSR nuclear DNA but not in cpDNA.