Date of Award
The Sigma-1 receptor (S1R) is an important pharmaceutical target that has been linked to several neurological diseases and drug addiction. It has been proposed that multimerization of S1R is important for attenuating its interactions with the dopamine transporter (DAT) and that S1R agonists promote dissociation to a monomeric form necessary for DAT interaction. This project aims to assess the role of the N-terminal transmembrane domain in oligomerization and binding activity of the mammalian Sigma-1 receptor (S1R). N-terminal truncations lacking the transmembrane domain and the E102Q variant, which displays altered activity and cellular localization, were expressed, purified in order to determine their contributions to the overall structure.
Koch, Christopher, "Sub-cloning, Expression, and Purification of the Wild Type and Mutated Sigma-1 Receptor" (2019). Undergraduate Honors Thesis Collection. 468.