Biology
Event Title
The Development of CRISPR Mediated Genetic Engineering in Candida viswanathii
Document Type
Poster Presentation
Location
Indianapolis, IN
Start Date
13-4-2018 2:30 PM
End Date
13-4-2018 4:00 PM
Sponsor
Douglas Bernstein (Ball State University), Irene Reizman (Rose-Hulman Institute of Technology)
Description
Candida viswanathii is a fungal species in the Candida clade. It is closely related to Candida tropicalis, a prominent human fungal pathogen. Using the Candida albicans CRISPR system as a template, we have begun to develop CRISPR mediated genetic engineering in C. viswanathii. We have identified guide sequences in the C. visawanathii ADE2 gene and cloned these sequences into a vector that also expresses Cas9. After generating these constructs, we cotransformed these vectors along with repair template that will introduce stop codons when incorporated into the C. viswanathii ADE2 locus. We were able to obtain dozens of transformants and we are now testing if these transformants have incorporated the repair template.
The Development of CRISPR Mediated Genetic Engineering in Candida viswanathii
Indianapolis, IN
Candida viswanathii is a fungal species in the Candida clade. It is closely related to Candida tropicalis, a prominent human fungal pathogen. Using the Candida albicans CRISPR system as a template, we have begun to develop CRISPR mediated genetic engineering in C. viswanathii. We have identified guide sequences in the C. visawanathii ADE2 gene and cloned these sequences into a vector that also expresses Cas9. After generating these constructs, we cotransformed these vectors along with repair template that will introduce stop codons when incorporated into the C. viswanathii ADE2 locus. We were able to obtain dozens of transformants and we are now testing if these transformants have incorporated the repair template.