Enhanced Cell Cycle Progression and Down Regulation of p21Cip1/Waf1 by PRL Tyrosine Phosphatases

Authors

Sean R. Werner
Paul A. Lee
Matthew W. DeCamp
Dring N. Crowell
Stephen K. Randall
Pamela L. Crowell, Butler UniversityFollow

Document Type

Article

Publication Date

2003

Publication Title

Cancer Letters

First Page

201

Last Page

211

DOI

http://dx.doi.org/10.1016/S0304-3835(03)00517-2

Abstract

Human PRL-1, PRL-2, and PRL-3 tyrosine phosphatases induce the malignant transformation of epithelial cells. We tested the hypothesis that the oncogenic effects of PRL occur by increasing cellular proliferation. Cells stably transfected with PRL-1 or PRL-2 exhibited 2.7–3.3-fold increases over control cells in the rate of DNA synthesis and the proportion of cells in S-phase, and they progressed more rapidly from G1 into S. In addition, cells overexpressing either PRL-1 or PRL-2 exhibited enhanced cyclin-dependent kinase 2 (CDK2) activity and significantly lower p21^Cip1/Waf1 protein levels, and PRL-1 overexpressing cells had higher cyclin A protein levels than control cells. We conclude that PRL phosphatases increase cell proliferation by stimulating progression from G1 into S phase, and this process may be dependent on the down regulation of the cyclin dependent kinase inhibitor p21^Cip1/Waf1.

Rights

Link leads to full text provided by Elsevier.

Recommended Citation

Werner, Sean R.; Lee, Paul A.; DeCamp, Matthew W.; Crowell, Dring N.; Randall, Stephen K.; and Crowell, Pamela L., "Enhanced Cell Cycle Progression and Down Regulation of p21Cip1/Waf1 by PRL Tyrosine Phosphatases" (2003). Scholarship and Professional Work – COPHS. 134.
https://digitalcommons.butler.edu/cophs_papers/134