Cell Cycle-Dependent Binding of Yeast Heat Shock Factor to Nucleosomes

Authors

Christina Bourgeois Venturi
Alexander M. Erkine, Butler UniversityFollow
David S. Gross

Document Type

Article

Publication Date

2000

Publication Title

Molecular and Cellular Biology

First Page

6435

Last Page

6448

DOI

http://dx.doi.org/10.1128/MCB.20.17.6435-6448.2000

Abstract

In the nucleus, transcription factors must contend with the presence of chromatin in order to gain access to their cognate regulatory sequences. As most nuclear DNA is assembled into nucleosomes, activators must either invade a stable, preassembled nucleosome or preempt the formation of nucleosomes on newly replicated DNA, which is transiently free of histones. We have investigated the mechanism by which heat shock factor (HSF) binds to target nucleosomal heat shock elements (HSEs), using as our model a dinucleosomal heat shock promoter (hsp82-ΔHSE1). We find that activated HSF cannot bind a stable, sequence-positioned nucleosome in G₁-arrested cells. It can do so readily, however, following release from G₁ arrest or after the imposition of either an early S- or late G₂-phase arrest. Surprisingly, despite the S-phase requirement, HSF nucleosomal binding activity is restored in the absence of hsp82 replication. These results contrast with the prevailing paradigm for activator-nucleosome interactions and implicate a nonreplicative, S-phase-specific event as a prerequisite for HSF binding to nucleosomal sites in vivo.

Rights

This article was archived with permission from American Society for Microbiology, all rights reserved. Document also available from Molecular and Cellular Biology.

Recommended Citation

Venturi, Christina Bourgeois; Erkine, Alexander M.; and Gross, David S., "Cell Cycle-Dependent Binding of Yeast Heat Shock Factor to Nucleosomes" (2000). Scholarship and Professional Work – COPHS. 142.
https://digitalcommons.butler.edu/cophs_papers/142