Biology

The Development of CRISPR Mediated Genetic Engineering in Candida viswanathii

Presenter Information

Elena North, Ball State University

Document Type

Poster Presentation

Location

Indianapolis, IN

Start Date

13-4-2018 2:30 PM

End Date

13-4-2018 4:00 PM

Description

Candida viswanathii is a fungal species in the Candida clade. It is closely related to Candida tropicalis, a prominent human fungal pathogen. Using the Candida albicans CRISPR system as a template, we have begun to develop CRISPR mediated genetic engineering in C. viswanathii. We have identified guide sequences in the C. visawanathii ADE2 gene and cloned these sequences into a vector that also expresses Cas9. After generating these constructs, we cotransformed these vectors along with repair template that will introduce stop codons when incorporated into the C. viswanathii ADE2 locus. We were able to obtain dozens of transformants and we are now testing if these transformants have incorporated the repair template.

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Apr 13th, 2:30 PM Apr 13th, 4:00 PM

The Development of CRISPR Mediated Genetic Engineering in Candida viswanathii

Indianapolis, IN

Candida viswanathii is a fungal species in the Candida clade. It is closely related to Candida tropicalis, a prominent human fungal pathogen. Using the Candida albicans CRISPR system as a template, we have begun to develop CRISPR mediated genetic engineering in C. viswanathii. We have identified guide sequences in the C. visawanathii ADE2 gene and cloned these sequences into a vector that also expresses Cas9. After generating these constructs, we cotransformed these vectors along with repair template that will introduce stop codons when incorporated into the C. viswanathii ADE2 locus. We were able to obtain dozens of transformants and we are now testing if these transformants have incorporated the repair template.