Chemistry

Listeria Detection by Super Paramagnetic Iron Oxide Nanoparticles

Presenter Information

Christian Shafer, Trine University

Document Type

Poster Presentation

Location

Indianapolis, IN

Subject Area

Chemistry

Start Date

13-4-2018 2:30 PM

End Date

13-4-2018 4:00 PM

Description

Superparamagnetic iron oxide nanoparticles (SPIONs) were investigated as a rapid detection method for Listeria. Synthesis of the SPIONs occurred using the coprecipitation method of aqueous iron salts. SPION cores were characterized by Dynamic Light Scattering (DLS), Scanning Electron Microscopy, and Atomic Force Microscopy. Coatings were applied to the core allowing for stabilization and functionalization of the SPIONs. The coatings used were starch or aminopropyl triethoxysilane and they were further functionalized using carnitine. Modifications of the core were confirmed using Thermogravimetric Analysis, DLS, and Infrared Spectroscopy. The binding of functionalized SPIONs and Listeria were then evaluated using Prussian Blue staining methods.

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Apr 13th, 2:30 PM Apr 13th, 4:00 PM

Listeria Detection by Super Paramagnetic Iron Oxide Nanoparticles

Indianapolis, IN

Superparamagnetic iron oxide nanoparticles (SPIONs) were investigated as a rapid detection method for Listeria. Synthesis of the SPIONs occurred using the coprecipitation method of aqueous iron salts. SPION cores were characterized by Dynamic Light Scattering (DLS), Scanning Electron Microscopy, and Atomic Force Microscopy. Coatings were applied to the core allowing for stabilization and functionalization of the SPIONs. The coatings used were starch or aminopropyl triethoxysilane and they were further functionalized using carnitine. Modifications of the core were confirmed using Thermogravimetric Analysis, DLS, and Infrared Spectroscopy. The binding of functionalized SPIONs and Listeria were then evaluated using Prussian Blue staining methods.