Chemistry
Event Title
Listeria Detection by Super Paramagnetic Iron Oxide Nanoparticles
Document Type
Poster Presentation
Location
Indianapolis, IN
Subject Area
Chemistry
Start Date
13-4-2018 2:30 PM
End Date
13-4-2018 4:00 PM
Sponsor
Steven Dulaney (Trine University)
Description
Superparamagnetic iron oxide nanoparticles (SPIONs) were investigated as a rapid detection method for Listeria. Synthesis of the SPIONs occurred using the coprecipitation method of aqueous iron salts. SPION cores were characterized by Dynamic Light Scattering (DLS), Scanning Electron Microscopy, and Atomic Force Microscopy. Coatings were applied to the core allowing for stabilization and functionalization of the SPIONs. The coatings used were starch or aminopropyl triethoxysilane and they were further functionalized using carnitine. Modifications of the core were confirmed using Thermogravimetric Analysis, DLS, and Infrared Spectroscopy. The binding of functionalized SPIONs and Listeria were then evaluated using Prussian Blue staining methods.
Listeria Detection by Super Paramagnetic Iron Oxide Nanoparticles
Indianapolis, IN
Superparamagnetic iron oxide nanoparticles (SPIONs) were investigated as a rapid detection method for Listeria. Synthesis of the SPIONs occurred using the coprecipitation method of aqueous iron salts. SPION cores were characterized by Dynamic Light Scattering (DLS), Scanning Electron Microscopy, and Atomic Force Microscopy. Coatings were applied to the core allowing for stabilization and functionalization of the SPIONs. The coatings used were starch or aminopropyl triethoxysilane and they were further functionalized using carnitine. Modifications of the core were confirmed using Thermogravimetric Analysis, DLS, and Infrared Spectroscopy. The binding of functionalized SPIONs and Listeria were then evaluated using Prussian Blue staining methods.