Biology & Sustainability
Intraspecific Variation in a Hawaiian Population of Ophioderma pendula (Ophioglossaceae)
Document Type
Oral Presentation
Location
Indianapolis, IN
Subject Area
Biology & Sustainability
Start Date
11-4-2014 9:45 AM
End Date
11-4-2014 10:30 AM
Sponsor
Warren Hauk (Denison University)
Description
Long-distance dispersal is the major means by which organisms colonize islands. Colonization of islands requires that species possess unique traits that facilitate rare, long-distance dispersal events for the establishment of populations. Populations established by a single, long-distance dispersal event typically contain relatively low amounts of genetic variation. Ophioderma pendula (L.) Presl is an epiphytic fern (a plant that grows on trees) in the adder's tongue fern family (Ophioglossaceae) that has colonized successfully many islands distributed across the Indian Ocean and Pacific Oceans. To evaluate within-population genetic variation of O. pendula from Hawaii, I analyzed nuclear ISSR variation from 30 individual sporophytes, and two chloroplast DNA (cpDNA) regions (trnH-psbA, rpoB-trnC) of five individuals from a single population. Analysis of ISSR variation from seven primers revealed 26 multi-locus genotypes among the 30 samples. However, sequences for both cpDNA regions showed no variation across the five individuals sampled. Comparisons to cpDNA sequences from a sample of O. pendula from the Philippines are consistent with the hypothesis that this Hawaiian population originated from a single colonization event followed by mutation in ISSR nuclear DNA but not in cpDNA.
Intraspecific Variation in a Hawaiian Population of Ophioderma pendula (Ophioglossaceae)
Indianapolis, IN
Long-distance dispersal is the major means by which organisms colonize islands. Colonization of islands requires that species possess unique traits that facilitate rare, long-distance dispersal events for the establishment of populations. Populations established by a single, long-distance dispersal event typically contain relatively low amounts of genetic variation. Ophioderma pendula (L.) Presl is an epiphytic fern (a plant that grows on trees) in the adder's tongue fern family (Ophioglossaceae) that has colonized successfully many islands distributed across the Indian Ocean and Pacific Oceans. To evaluate within-population genetic variation of O. pendula from Hawaii, I analyzed nuclear ISSR variation from 30 individual sporophytes, and two chloroplast DNA (cpDNA) regions (trnH-psbA, rpoB-trnC) of five individuals from a single population. Analysis of ISSR variation from seven primers revealed 26 multi-locus genotypes among the 30 samples. However, sequences for both cpDNA regions showed no variation across the five individuals sampled. Comparisons to cpDNA sequences from a sample of O. pendula from the Philippines are consistent with the hypothesis that this Hawaiian population originated from a single colonization event followed by mutation in ISSR nuclear DNA but not in cpDNA.