Biology & Sustainability
The Prevalence of E.coli and Fecal Coliforms on Equestrian Workers’ Boots in Central Kentucky
Document Type
Oral Presentation
Location
Indianapolis, IN
Subject Area
Biology & Sustainability
Start Date
11-4-2014 10:45 AM
End Date
11-4-2014 11:45 AM
Sponsor
Beverly Juett (Midway College)
Description
Forty-five samples were taken from equine workers' boots at 4 horse farms in central Kentucky. E.coli was isolated from 24 samples (53.3%) and fecal coliforms were isolated from 42 (93.3%) out of the 45 total samples collected. Sterile swabs were used to collect the samples from the boots. The swabs were placed in Stuarts transport medium and arrived at the lab within 2 hours. Upon arrival the samples were streaked for isolation on Coliscan Easygel© plates under a laminar air flow hood. The inoculated plates were placed in an incubator at 35º C and read for presence or absence of E.coli and/or fecal coliforms at 24 and 48 hours. E.coli and fecal coliforms found on the boots may be a possible source of secondary infections in the communities.
The Prevalence of E.coli and Fecal Coliforms on Equestrian Workers’ Boots in Central Kentucky
Indianapolis, IN
Forty-five samples were taken from equine workers' boots at 4 horse farms in central Kentucky. E.coli was isolated from 24 samples (53.3%) and fecal coliforms were isolated from 42 (93.3%) out of the 45 total samples collected. Sterile swabs were used to collect the samples from the boots. The swabs were placed in Stuarts transport medium and arrived at the lab within 2 hours. Upon arrival the samples were streaked for isolation on Coliscan Easygel© plates under a laminar air flow hood. The inoculated plates were placed in an incubator at 35º C and read for presence or absence of E.coli and/or fecal coliforms at 24 and 48 hours. E.coli and fecal coliforms found on the boots may be a possible source of secondary infections in the communities.