Chemistry
Functionalizing Fluorescent Protein for Detection of Explosive Compounds in Hydrogels
Document Type
Oral Presentation
Location
Indianapolis, IN
Subject Area
Chemistry
Start Date
11-4-2014 11:00 AM
End Date
11-4-2014 11:45 AM
Sponsor
Scott Kennedy (Anderson University)
Description
Detecting explosive materials is a major concern in matters of national and individual security. It is very important to be able to detect these materials with high sensitivity and selectivity. We are exploring a fluorescently functionalized hydrogel system to address these requirements. Red, yellow, and green fluorescent proteins are expressed by recombinant E.coli and purified using butyl-sepharose chromatography columns. Fluorescent proteins are functionalized via MSH, a reagent that oxidatively eliminates cysteine residues into dehydroalanine. Functionalized proteins are then copolymerized into polyacrylamide hydrogels as confirmed by electroelution experiments.
Functionalizing Fluorescent Protein for Detection of Explosive Compounds in Hydrogels
Indianapolis, IN
Detecting explosive materials is a major concern in matters of national and individual security. It is very important to be able to detect these materials with high sensitivity and selectivity. We are exploring a fluorescently functionalized hydrogel system to address these requirements. Red, yellow, and green fluorescent proteins are expressed by recombinant E.coli and purified using butyl-sepharose chromatography columns. Fluorescent proteins are functionalized via MSH, a reagent that oxidatively eliminates cysteine residues into dehydroalanine. Functionalized proteins are then copolymerized into polyacrylamide hydrogels as confirmed by electroelution experiments.