Psychology
The Effects of Ethanol on Spatial Memory in cHAP Mice
Document Type
Oral Presentation
Location
Indianapolis, IN
Subject Area
Psychology
Start Date
11-4-2014 1:00 PM
End Date
11-4-2014 2:15 PM
Sponsor
Alexis Green (Hanover College)
Description
Background: The aim of this study is to find out whether abusing alcohol has any effect on spatial memory in selectively-bred high alcohol preferring (cHAP) mice.
Method: Experiment 1: sixteen mice were divided equally into an acute ethanol group and a control group. Each group was given 2.0g/kg subcutaneous injections of 20% ethanol in an isotonic saline solution or an equivalent volume of isotonic saline solution twice a day for one day. Following, each mouse was run independently in Morris Water Maze for five days for three trials per day. After trials were completed, each group was given a challenge trial in which the platform was removed. Experiment 2: fifteen mice were divided equally into a chronic ethanol group and a control group. They were given 2.0g/kg subcutaneous injections of 20% ethanol in an isotonic saline solution or an equivalent volume of isotonic saline solution twice a day for fourteen days. Following, these mice were run in a Morris Water Maze using the same method as the acute group.
Results: We expect to find that the chronic exposure group will have the slowest rates of acquisition and will spend the least amount of time in the platform quadrant during the challenge trial.
Conclusion: We expect that all subjects will be able to improve upon their times of finding the platform; however, we expect the chronic exposure group to have significantly slower acquisition times when compared to the other groups.
The Effects of Ethanol on Spatial Memory in cHAP Mice
Indianapolis, IN
Background: The aim of this study is to find out whether abusing alcohol has any effect on spatial memory in selectively-bred high alcohol preferring (cHAP) mice.
Method: Experiment 1: sixteen mice were divided equally into an acute ethanol group and a control group. Each group was given 2.0g/kg subcutaneous injections of 20% ethanol in an isotonic saline solution or an equivalent volume of isotonic saline solution twice a day for one day. Following, each mouse was run independently in Morris Water Maze for five days for three trials per day. After trials were completed, each group was given a challenge trial in which the platform was removed. Experiment 2: fifteen mice were divided equally into a chronic ethanol group and a control group. They were given 2.0g/kg subcutaneous injections of 20% ethanol in an isotonic saline solution or an equivalent volume of isotonic saline solution twice a day for fourteen days. Following, these mice were run in a Morris Water Maze using the same method as the acute group.
Results: We expect to find that the chronic exposure group will have the slowest rates of acquisition and will spend the least amount of time in the platform quadrant during the challenge trial.
Conclusion: We expect that all subjects will be able to improve upon their times of finding the platform; however, we expect the chronic exposure group to have significantly slower acquisition times when compared to the other groups.